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Perichondrium-Wrapped Collagenous Matrices to Induce Chondroneogenesis
An In Vitro Study
Jeroen W. L. van Lange, MD;
Kirsten de Roo, MD;
Esther Middelkoop, PhD;
Theo van den Bos, PhD;
Vincent Everts, PhD;
Gilbert J. Nolst Trenité, MD, PhD
Arch Facial Plast Surg. 2001;3:122-126.
Objective To develop a model for cartilage regeneration in vitro, to be used for
cartilage reconstruction in vivo.
Methods Collagenous matrices were wrapped in a perichondrium layer. The matrices
served as carriers to allow migration of cells from the perichondrium into
the matrix. Culture conditions stimulated cell growth and proliferation.
Results After 4 weeks of culturing, microscopic examination showed an increase
of cell layers around the matrices but also of cells migrated into porous
matrices. Immunohistochemical staining of these cultured cells expressed type
II collagen intracellularly.
Conclusions This model seems appropriate to culture mucoperichondrial explants in
combination with collagenous matrices. Cells migrate into the pores of the
matrix, survive, and synthesize matrix components. Actual formation of cartilage
has not been shown to occur. Adding growth factors to this model may influence
induction of this activity.
From the Departments of Otorhinolaryngology (Drs van Lange, de Roo,
and Nolst Trenité), Dermatology (Dr Middelkoop), Periodontology, Academical
Center of Dentistry–Amsterdam (Drs van den Bos and Everts), and Cell
Biology and Histology (Dr Everts), Academical Medical Center, University of
Amsterdam, Amsterdam, the Netherlands.
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