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The Effect of Copper Tripeptide and Tretinoin on Growth Factor Production in a Serum-Free Fibroblast Model
Matthew C. McCormack, BA;
Kenneth C. Nowak, MD;
R. James Koch, MD, MS
Arch Facial Plast Surg. 2001;3:28-32.
Objective To evaluate the effect of copper tripeptide and tretinoin on normal
and keloid-producing dermal fibroblasts in a serum-free in vitro model. The
cellular response was described in terms of viability and secretion of basic
fibroblast growth factor (bFGF) and transforming growth factor-ß1 (TGF-ß1).
Methods Primary cell lines were established from patient facial skin samples
obtained during surgery and plated in serum-free media. At 0 hour, copper
tripeptide (1 x 10 -9 mol/L), tretinoin (1 x
10 -5 mol/L), or appropriate control vehicle was added. Cell
counts and viability were established at 24, 72, and 120 hours. Supernatants
were collected at the same intervals and were assessed for bFGF and TGF-ß1
concentrations using the enzyme-linked immunosorbent assay technique.
Results Cell lines showed viability between 86% and 96% (mean, 92%) throughout
the experiment. Tretinoin-treated normal fibroblasts secreted more bFGF than
did controls at 24 hours (P<.05). Tretinoin-treated
keloid-producing fibroblasts secreted more TGF-ß1 than did controls at
120 hours (P<.05). Keloid-producing fibroblasts
treated with copper tripeptide secreted less TGF-ß1 than did controls
at 24 hours (P<.05); a similar trend was observed
in normal fibroblasts.
Conclusions Normal fibroblasts treated with tretinoin produced more bFGF than did
controls, and this might partially explain the clinically observed tightening
effects of tretinoin. Normal and keloid-producing dermal fibroblasts treated
with copper tripeptide secreted less TGF-ß1 than did controls, suggesting
a possible clinical use for decreasing excessive scar formation.
From the Wound Healing and Tissue Engineering Laboratory, Division
of Otolaryngology–Head and Neck Surgery, Stanford University Medical
Center, Stanford, Calif.
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
Effects of Copper Tripeptide on the Growth and Expression of Growth Factors by Normal and Irradiated Fibroblasts
Pollard et al.
Arch Facial Plast Surg 2005;7:27-31.
ABSTRACT
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